Lynn Poole points out that science is often used in crime detection, as when Johns Hopkins physicist Robert W. Wood used ultra violet light to help police solve such crimes as the "candy box murder." Lieutenant Nelligan, Director of the Baltimore City Crime Lab, says that a criminalist, or scientific crime detective, requires a background in both science and police techniques. A dramatization follows the investigation of a fictitious crime, a burglary in a suburban house, to demonstrate the basic tools used to solve the crime: a microscope to match hairs and fibers, a spectrograph to analyze metals and glass, a vacuum cleaner to pick up evidence in a special filter, and a magnifying glass to enlarge details. The density gradient test is also demonstrated and explained. The cast for the scene is from Baltimore's Hilltop Theater: Caddell Burroughs, Elaine Swann, Earl Simmons, John Holland, Alex Quiroga, and Buff Shurr.

This program opens with a visual of the sound waves of Lynn Poole's voice as they appear on an oscilloscope. A brief film shows some of the glass tools that are basic to scientific research. Frank Witt, a Johns Hopkins University graduate student in electrical engineering, then explains the design and function of a cathode ray oscilloscope. He shows how to solve a simple electrical problem using this oscilloscope and comments that its applications make it one of science's most versatile tools. Chemistry graduate student Bernard Blaustein discusses the process, history, applications, and importance of distillation. He also explains equipment used to separate liquid from a dye using distillation. After a brief film on the history of microscopes, Dr. Dethier explains the parts of a microscope and their functions. He then offers a microscopic view of both a butterfly's wing and a snow flea. At the conclusion of the show, Lynn Poole asks viewers to send him what they consider to be the greatest achievement in science in 1953.

Lynn Poole explains that "microscope" is from the two Greek words "mikros," small, and "skopos," a watcher. He notes that Dutch Antony Van Leeuwenhoek and English Robert Hooke were both instrumental in the development of the instrument and that Charles A. Spencer was America's first microscope maker. Dr. Schwartz, using the RCA Vidicon (a microscope connected to a television monitor), shows slides of water specimens from ponds in New York and New Jersey. The organisms he identifies include one-celled blepharisma and stentor, which he compares to the multi-celled rotifer, the plant spirogyra, diatoms, and the beating heart of a daphnia or water flea. Dr. Schwartz also shows a replica of Van Leeuwenhoek's microscope and his drawings of bacteria, and he demonstrates how to make a slide for viewing.

Two seemingly unrelated crimes are linked and subsequently solved through forensic techniques involving spectrometry, blood typing, and ballistics with the use of microscopic methods.

The need to see cells in motion in high magnification led to the development of the television microscope.